Part:BBa_K1439002:Design

This part contains a reporter gene BBa_J04450, combined with OriTR. Used to test plasmid mobility.

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 117
  Illegal NgoMIV site found at 127
  Illegal AgeI site found at 1160
  Illegal AgeI site found at 1272
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI site found at 19
  

Design Notes

OriTR region contains some promoters, we put it in the back of reporter gene . In order to reduce the impact on protein expression

Source

OriTR comes form BBa_J01003, reporter gene comes from BBa_J04450.